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Titlebook: Mesenchymal Stem Cells; Methods and Protocol Massimiliano Gnecchi Book 2016Latest edition Springer Science+Business Media New York 2016 Mes

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书目名称Mesenchymal Stem Cells
副标题Methods and Protocol
编辑Massimiliano Gnecchi
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Mesenchymal Stem Cells; Methods and Protocol Massimiliano Gnecchi Book 2016Latest edition Springer Science+Business Media New York 2016 Mes
描述.This volume aims to outline the current status of the Mesenchymal Stem Cells.(.MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than ever, “under the spotlight”. .Mesenchymal Stem Cells: Methods and Protocols, Second Edition. is organized into four sections. The first guides the reader through a series of state-of-the-art reviews summarizing the use of MSC for the treatment of various diseases. The other three sections are a collection of methodological chapters covering several aspects: isolation and characterization of MSC; expansion of MSC for clinical use; production and characterization of the MSC secretome. Written in the highly successful Methods in Molecular Biology series format, the method chapters include introductions to their respective topics, complete lists of the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting which will help the researcher to avoid known pitfalls...Authoritative and cutting-edge, .Mesenchymal Stem Cells: Methods and Protocols, Second Edition., aims to ensure suc
出版日期Book 2016Latest edition
关键词Mesenchymal stem cells (MSC); cell therapy; isolation; characterization; MSC secretome; clinical use
版次2
doihttps://doi.org/10.1007/978-1-4939-3584-0
isbn_softcover978-1-4939-8085-7
isbn_ebook978-1-4939-3584-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Isolation, Expansion, and Immortalization of Human Adipose-Derived Mesenchymal Stromal Cells from Bive reliable and reproducible results. Here, we describe a protocol to isolate and expand human adipose stromal cells from specimens obtained from tissue biopsies and liposuction surgical interventions. Finally, we broadly describe the cell immortalization technique, and particular attention is paid to some of the apparently “secondary” aspects.
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Mesenchymal Stromal Cells in Hematopoietic Stem Cell Transplantationafe; moreover, promising preliminary results in terms of efficacy have been reported in some clinical trials. This chapter focuses on recent advances in MSC therapy by reporting on the most important relevant studies in the field of HSCT.
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he theoretical rules given in these books are routinely used by physicists to compute observable quantities. Their predictions can then be compared with experimental data. There is no fundamental disagreement among physicists on how to use the theory for these practical purposes. However, there are
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