Titlebook: Membrane Proteins Production for Structural Analysis; Isabelle Mus-Veteau Book 2014 Springer Science+Business Media New York 2014 amphipol

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Membrane Protein Production for Structural Analysis,he advances in heterologous expression systems, stabilization tools, and structural methods that contributed to the growing number of recombinant mammalian integral MP structures solved this past years.

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Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studie generating hydrophobic environments, modifications, or labeling of membrane proteins open fascinating perspectives for structural approaches, biochemical studies and throughput screening applications. We provide an overview on the relevant toolbox for the quality optimization of membrane proteins a

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Overexpression of Membrane Proteins in , for Structural and Functional Studies: A Focus on the Rabbs overexpressed in . are presented and the methodologies used to obtain these structures are discussed. It also examines the case of the rabbit Ca.-ATPase Serca1a, which was expressed in . in our laboratory, and describes the critical steps that led to the determination of the three-dimensional stru

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,New Amphiphiles to Handle Membrane Proteins: “Ménage à Trois” Between Chemistry, Physical Chemistrynt .-surfactant families. We then focus on the design and the surface and self-aggregation properties of two recent series—including some original compounds—of .-surfactants bearing branched polar heads. The promising biochemical applications of .-surfactants, from the literature from 2010, are revi

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Structural Studies of TSPO, a Mitochondrial Membrane Protein,sted that TSPO could form homopolymers within heteropolymer complexes. Because of the low native abundance of TSPO, production of recombinant TSPO is a first step for any structural study. In this chapter, we present the current understanding of TSPO overexpression studies in bacteria, purification

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Jean-Louis Banèresor contribute to future technical advances in the field. Each chapter has practical exercises to enable readers to check their progress. A full glossary of technical terms used is included..978-1-84628-766-4Series ISSN 1863-7310 Series E-ISSN 2197-1781