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Titlebook: Membrane Proteins; Isolation and Charac Angelo Azzi,Lanfranco Masotti,Arnaldo Vecli Book 1986 Springer-Verlag Berlin Heidelberg 1986 ATP.Li

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se entitled Isolation and Characterization of Membrane Proteins: Biochemical and Bio­ physical Aspects sponsored by the Federation of European Biochemical So­ cieties (FEBS) and the Italian Research Council (CNR). The success of the course and the continuous development in the field of membrane biol
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Use of Fluorescent Probes of the Adenine Nucleotide Carrier for Binding Studies and Analysis of Confen used extensively to study the mechanism of ADP/ATP transport. The fact that CATR binds to the carrier from the outside of the mitochondrial membrane and BA from the inside points to the asymmetric orientation of the carrier in the membrane (for review, see Vignais et al. 1985).
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Molecular Weight Estimation of Membrane Proteins: Gel Permeation Chromatography and Sucrose Gradient be interpreted carefully, considering especially the asymmetry of the protein complex, the possible interaction of the detergent with the gel matrix, and the influence of other components of the medium (e.g., pH, ionic strength).
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Membrane Signal Transduction via Protein Kinase C). The binding of radioactive phorbol esters can be inhibited competitively by diacylglycerols (Sharkey et al. 1984, Sharkey and Blumberg 1985), suggesting that diacylglycerol and phorbol esters have the same binding site.
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