Titlebook: Membrane Proteins; Isolation and Charac Angelo Azzi,Lanfranco Masotti,Arnaldo Vecli Book 1986 Springer-Verlag Berlin Heidelberg 1986 ATP.Li

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a very popular hardcover edition, published in 1999. An account is given of the state of the art of numerical methods employed in computational fluid dynamics. Numerical principles are treated in detail, using elementary methods. Attention is given to difficulties arising from geometric complexity o

Legion

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Molecular Weight Estimation of Membrane Proteins: Gel Permeation Chromatography and Sucrose Gradientsoluble protein, this can be done easily and accurately by a gel filtration expe ntn, in which the permeation of the protein into the pores of a gel matrix is compared with that of proteins of known size and molecular weight. A solubilized membrane protein, however, is a detergent-protein-complex, w

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The Problem of Light Scattering in the Absorbance and Fluorescence Studies of Proteins in Membranesosphorylation, electron transport, etc., and changes in the osmolarity of the medium can modify the scattering properties of mitochondria. Presumably the changes in scattering properties reflect changes in particle conformation (Latimer et al. 1968).

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Reproducible Preparation of Phosphatidylserine Vesicles for Fluorescence Studies of Protein Incorporrotein or enzyme with the lipids, the protein is isolated and purified and then reconstituted with model lipid systems. Hence it is vital that one achieves a reproducible preparation of lipid vesicles. The presence of contaminants will alter the properties of the lipid matrix. Lipids isolated from n

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Purification and Reconstitution of the Proton Translocating Membrane Bound Inorganic Pyrophosphataseynthesis is coupled to light-induced electron transport in a manner analogous to ATP synthesis (Baltscheffsky et al. 1966) and the enzyme catalyzing this reaction is a H.-translocating, membrane-bound PPase (Baltscheffsky 1967). The PPi synthesis is a reversible reaction and when PPi is hydrolyzed,

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Membrane Signal Transduction via Protein Kinase C4). Many different activators act via this pathway. PKC is activated by diacylglycerols that are produced by a phospholipase C as a consequence of the binding of an activator to a receptor. The activated PKC then phosphorylates intracellular substrates, which lead to the responses of the cell to the

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Extraction, Partial Purification, and Reconstitution of a Mixture of Carriers from the Inner Mitochotion of the inner mitochondrial membrane will be followed by the reconstitution of the solubilized proteins either directly or after a partial purification by hydroxylapatite chromatography. Subsequently the carrier-mediated accumulation of a substrate will be measured.

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Polypeptide-Lipid Interactions as Studied by 13C NMRand function (Burnell et al. 1981, de Krujiff and Cullis 1980, Pink et al. 1981, Spisni et al. 1979, Tamm and Seelig 1983). Gramicidin A (GA), due to its highly hydrophobic character, is a polypentadecapeptide able to incorporate in lipid bilayers forming helical structures (Urry et al. 1979a,b, Wal

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Conformational Changes in Polypeptides and Proteins Brought About by Interactions with Lipidsrecognition, quantitative determination and monitoring of the changes of such structures is of great importance in understanding the relationships between structure and function of biological macromolecules.