Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Jennifer J. Kohler,Steven M. Patrie Book 2013 Springer Science+Business Media, LLC

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Characterization of Membrane-Associated Glycoproteins Using Lectin Affinity Chromatography and Mass finity chromatography is one of the most widely used approaches for enrichment of glycoproteins at the protein level. Here, we describe a strategy for the characterization of membrane glycoproteins including membrane protein extraction, lectin affinity chromatography, protein digestion, and analysis

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In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysisf glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental workflow. This chapter outlines an optimized in-solution digestion protocol to prepare samples

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Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionizatio electrospray ionization (ESI) mass spectrometry (MS), CE enables fast, sensitive, and efficient separations for the accurate identification of a large variety of glycoform mixture types. In this chapter several reliable off- and on-line CE-based methods for the analysis of glycoforms with ESI MS/MS

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Structural Separations by Ion Mobility-MS for Glycomics and Glycoproteomicsycoconjugates. IM-MS provides separations in two dimensions; one on the basis of molecular surface area or structure, and the other on molecular mass which creates the ability to differentiate biomolecular classes and isobaric species. When applied to the characterization of glycoproteins, IM-MS sep

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Stable Isotope Labeling of N-Glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometrl glycosylation is also associated with various pathologies, including cancer, and inflammatory and degenerative diseases, technology for comprehensive analysis of glycoproteins, or glycoproteomics, is important not only for biological studies but also for biomedical and clinical research, including

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