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Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Jennifer J. Kohler,Steven M. Patrie Book 2013 Springer Science+Business Media, LLC

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发表于 2025-3-21 16:43:08 | 显示全部楼层 |阅读模式
书目名称Mass Spectrometry of Glycoproteins
副标题Methods and Protocol
编辑Jennifer J. Kohler,Steven M. Patrie
视频video
概述Resource for those who work at the interface of glycobiology and mass spectrometry.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
丛书名称Methods in Molecular Biology
图书封面Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Jennifer J. Kohler,Steven M. Patrie Book 2013 Springer Science+Business Media, LLC
描述. Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In .Mass Spectrometry of Glycoproteins: Methods and Protocols, .expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.. .Authoritative and practical, .Mass Spectrometry of Glycoproteins: Methods and Protocol . is  an essential resource for those who work at the interface of glycobiolog
出版日期Book 2013
关键词Glycosylation; associated ionization; chromatographic strategies; fragmentation methods; glyco-specific
版次1
doihttps://doi.org/10.1007/978-1-62703-146-2
isbn_softcover978-1-4939-6203-7
isbn_ebook978-1-62703-146-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2013
The information of publication is updating

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Mass Spectrometry of Glycoproteins978-1-62703-146-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Characterization of Membrane-Associated Glycoproteins Using Lectin Affinity Chromatography and Mass finity chromatography is one of the most widely used approaches for enrichment of glycoproteins at the protein level. Here, we describe a strategy for the characterization of membrane glycoproteins including membrane protein extraction, lectin affinity chromatography, protein digestion, and analysis by LC-MS/MS.
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In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysisf glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental workflow. This chapter outlines an optimized in-solution digestion protocol to prepare samples for glycopeptide-based mass analysis.
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Jennifer J. Kohler,Steven M. PatrieResource for those who work at the interface of glycobiology and mass spectrometry.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
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Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/625277.jpg
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Sialic Acid Capture-and-Release and LC-MSn Analysis of Glycopeptidesthe peptide backbone with intact glycans still attached are used to pinpoint the glycosylation sites of glycopeptides containing several Ser/Thr residues. The step-by-step protocols for fragmentation analyses of O- and N-glycopeptides enriched from human cerebrospinal fluid are described.
发表于 2025-3-23 01:51:30 | 显示全部楼层
Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Cfor pre-fractionation of complex samples at the protein or peptide level. In this chapter we present a straightforward HILIC SPE enrichment technique and then combine C18 RP and HILIC enrichment for analysis of glycopeptides. Finally, we demonstrate HILIC enrichment using trifluoroacetic acid as an
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Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization-induced dissociation (CID) at low energies are also described. Ample sections are further devoted to on-line CE/ESI MS technique and its application to separation and identification of O-glycopeptides and CS/DS oligosaccharides. The concept and construction principles of two different sheathless C
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