Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Jennifer J. Kohler,Steven M. Patrie Book 2013 Springer Science+Business Media, LLC

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书目名称Mass Spectrometry of Glycoproteins影响因子(影响力)




书目名称Mass Spectrometry of Glycoproteins影响因子(影响力)学科排名




书目名称Mass Spectrometry of Glycoproteins网络公开度




书目名称Mass Spectrometry of Glycoproteins网络公开度学科排名




书目名称Mass Spectrometry of Glycoproteins被引频次




书目名称Mass Spectrometry of Glycoproteins被引频次学科排名




书目名称Mass Spectrometry of Glycoproteins年度引用




书目名称Mass Spectrometry of Glycoproteins年度引用学科排名




书目名称Mass Spectrometry of Glycoproteins读者反馈




书目名称Mass Spectrometry of Glycoproteins读者反馈学科排名

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Mass Spectrometry of Glycoproteins978-1-62703-146-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Characterization of Membrane-Associated Glycoproteins Using Lectin Affinity Chromatography and Mass finity chromatography is one of the most widely used approaches for enrichment of glycoproteins at the protein level. Here, we describe a strategy for the characterization of membrane glycoproteins including membrane protein extraction, lectin affinity chromatography, protein digestion, and analysis by LC-MS/MS.

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In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysisf glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental workflow. This chapter outlines an optimized in-solution digestion protocol to prepare samples for glycopeptide-based mass analysis.

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Jennifer J. Kohler,Steven M. PatrieResource for those who work at the interface of glycobiology and mass spectrometry.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the

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Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/625277.jpg

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Sialic Acid Capture-and-Release and LC-MSn Analysis of Glycopeptidesthe peptide backbone with intact glycans still attached are used to pinpoint the glycosylation sites of glycopeptides containing several Ser/Thr residues. The step-by-step protocols for fragmentation analyses of O- and N-glycopeptides enriched from human cerebrospinal fluid are described.

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Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Cfor pre-fractionation of complex samples at the protein or peptide level. In this chapter we present a straightforward HILIC SPE enrichment technique and then combine C18 RP and HILIC enrichment for analysis of glycopeptides. Finally, we demonstrate HILIC enrichment using trifluoroacetic acid as an

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Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization-induced dissociation (CID) at low energies are also described. Ample sections are further devoted to on-line CE/ESI MS technique and its application to separation and identification of O-glycopeptides and CS/DS oligosaccharides. The concept and construction principles of two different sheathless C