书目名称 | Mass Spectrometry of Glycoproteins |
副标题 | Methods and Protocol |
编辑 | Jennifer J. Kohler,Steven M. Patrie |
视频video | |
概述 | Resource for those who work at the interface of glycobiology and mass spectrometry.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | . Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In .Mass Spectrometry of Glycoproteins: Methods and Protocols, .expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.. .Authoritative and practical, .Mass Spectrometry of Glycoproteins: Methods and Protocol . is an essential resource for those who work at the interface of glycobiolog |
出版日期 | Book 2013 |
关键词 | Glycosylation; associated ionization; chromatographic strategies; fragmentation methods; glyco-specific |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-62703-146-2 |
isbn_softcover | 978-1-4939-6203-7 |
isbn_ebook | 978-1-62703-146-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC 2013 |