Titlebook: Mass Spectrometry Imaging; Principles and Proto Stanislav S. Rubakhin,Jonathan V. Sweedler Book 2010 Springer Science+Business Media, LLC 2

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Elena S.F. Berman,Susan L. Fortson,Kristen S. Kulp

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A Mass Spectrometry Primer for Mass Spectrometry Imagingion to guidelines for choosing the most suitable MSI method for specific investigations, cross-references are provided to the chapters in this volume that describe the appropriate experimental protocols.

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Imaging of Metals, Metalloids, and Non-metals by Laser Ablation Inductively Coupled Plasma Mass Speceld (LA-ICP-SFMS) or quadrupole mass spectrometers (LA-ICP-QMS) to produce images of detailed regionally specific element distributions in thin biological tissue sections. Nowadays, MSI studies focus on brain research for studying neurodegenerative diseases such as Alzheimer’s or Parkinson’s, stroke

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The Application and Potential of Ion Mobility Mass Spectrometry in Imaging MS with a Focus on Lipidser ion mobility MALDI-MS ability to analyze various biomolecules in tissue, that is, lipids and proteins, as well as its ability to separate species from all of the major phospholipid classes from tissue and extracts, the effects that radyl chain length, degree of unsaturation, head group compositio

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Matrix-Assisted Laser Desorption/Ionization and Nanoparticle-Based Imaging Mass Spectrometry for Smayte migration during the matrix application process. To overcome these problems, we reported a nanoparticle (NP)-assisted laser desorption/ionization (nano-PALDI)-based IMS, in which the matrix crystallization process is eliminated. In this chapter, a practical protocol for MALDI-IMS of lipids is ou

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Preparation of Single Cells for Imaging Mass Spectrometryed from the cellular growth medium. Using this protocol, we achieve high-quality, reproducible IMS images from three diverse cell types: MCF7 human breast cancer cells, Madin-Darby canine kidney (MDCK) cells, and NIH/3T3 mouse fibroblasts. This preparation method allows rapid and routine IMS analysi

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Applying Imaging ToF-SIMS and PCA in Differentiation of Tissue Typesprincipal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra.

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Book 2010ions are described in detail.Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures.Also included are