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Titlebook: Manual of Antisense Methodology; Gunther Hartmann,Stefan Endres Book 1999 Springer Science+Business Media New York 1999 Nucleotide.cell cu

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发表于 2025-3-21 19:33:02 | 显示全部楼层 |阅读模式
书目名称Manual of Antisense Methodology
编辑Gunther Hartmann,Stefan Endres
视频video
丛书名称Perspectives in Antisense Science
图书封面Titlebook: Manual of Antisense Methodology;  Gunther Hartmann,Stefan Endres Book 1999 Springer Science+Business Media New York 1999 Nucleotide.cell cu
描述In the past few years, antisense methodology has moved from.in vitro. studies to .in vivo. studies and first humantrials. While the basic concept of antisense technology is simple, themethodological problems associated with its use are numerous andcomplex. Antisense- based methods have proven to be a field ofresearch where careful attention to experimental protocols andappropriate controls is necessary. .The .Manual of Antisense Methodology. emphasizes the applicationof antisense oligonucleotides, and is a guide for the identificationof antisense and non-antisense effects in different experimentalsettings. The work is organized into three sections: antisenseapplication .in vitro., antisense application .in vivo.(animal models) and finally, clinical antisense studies. Where at allpossible, the methods are described in sufficient detail to allowreproduction of a given experiment. .The .Manual of Antisense Methodology. will be of interest toresearchers in immunology, cancer research, pharmacology and internalmedicine; and physicians conducting clinical studies in these fields.
出版日期Book 1999
关键词Nucleotide; cell culture; chemistry; delivery; drugs; immunology; pharmacology; receptor; reproduction; synth
版次1
doihttps://doi.org/10.1007/978-1-4615-5067-9
isbn_softcover978-1-4613-7304-9
isbn_ebook978-1-4615-5067-9Series ISSN 1566-0389
issn_series 1566-0389
copyrightSpringer Science+Business Media New York 1999
The information of publication is updating

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Inhibition of TNF Synthesis by Antisense Oligonucleotidesalso causes upregulation of adhesion molecules on neutrophils and endothelial cells, enhancement of vascular permeability (Beutler et al., 1989; Leeper-Woodford et al., 1991; Tracey et al., 1988), and stimulation of neutrophil respiratory burst activity (Nathan, 1987).
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1566-0389 ncept of antisense technology is simple, themethodological problems associated with its use are numerous andcomplex. Antisense- based methods have proven to be a field ofresearch where careful attention to experimental protocols andappropriate controls is necessary. .The .Manual of Antisense Methodo
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How to Choose Optimal Antisense Targets in an mRNArmed in translation arrest assays and in cell culture. Sequences with well-known non-antisense effects have to be avoided. Finally, stringent controls are required to prove an antisense-mediated mechanism of action.
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How to Exclude Immunostimmulatory and Other Nonantisense Effects of Antisense Oligonucleotidessense mechanism of action. These nonantisense activities, which can confound experimental interpretation, may be avoided through understanding the sequence motifs and responsible mechanisms, and through the careful use of appropriate control oligonucleotides.
发表于 2025-3-22 19:54:36 | 显示全部楼层
Book 1999ntisense technology is simple, themethodological problems associated with its use are numerous andcomplex. Antisense- based methods have proven to be a field ofresearch where careful attention to experimental protocols andappropriate controls is necessary. .The .Manual of Antisense Methodology. emph
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Lipid-Based Carriers for the Systemic Delivery of Antisense Drugsulated) oligonucleotide, and there is mounting evidence at the preclinical and clinical levels that antisense drugs are efficacious against a variety of diseases. Consequently, it might be asked“What is the rationale for the use of carriers to deliver antisense oligonucleotides .?”.
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Yogesh S. Sanghvi,Mark Andrade,Rantjit R. Deshmukh,Lars Holmberg,Anthony N. Scozzari,Douglas L. Coleection between the golden ratio (the “divine proportion" used by Phidias and many other artists and enshrined in Leonardo‘s Vitruvian Man, shown on the front cover), and the Fibonacci spiral (observable in botany, e.g., in the placement of sunflower seeds); is the coast of Tuscany infinitely long?;
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Bernhard Schu,Heinrich Brinkmeier is proven; without using terms like "one may show that ... .Mathematics plays an important role in mechanics and other human endeavours. Validating examples in this first volume include, for instance: the connection between the golden ratio (the “divine proportion" used by Phidias and many other ar
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