Titlebook: Lysosomes; Methods and Protocol Karin Öllinger,Hanna Appelqvist Book 2017 Springer Science+Business Media LLC 2017 apoptosis.cell repair.de

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书目名称Lysosomes影响因子(影响力)




书目名称Lysosomes影响因子(影响力)学科排名




书目名称Lysosomes网络公开度




书目名称Lysosomes网络公开度学科排名




书目名称Lysosomes被引频次




书目名称Lysosomes被引频次学科排名




书目名称Lysosomes年度引用




书目名称Lysosomes年度引用学科排名




书目名称Lysosomes读者反馈




书目名称Lysosomes读者反馈学科排名

消极词汇

Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes,d cells using highly specific monoclonal antibodies and fluorescence microscopy. The main advantage of this method is its sensitivity, and the fact that it provides spatial information on lysosomal exocytosis at the single cell level.

露天历史剧

EXPEL

钢盔

Analysis of ,- and ,-Glycosylation of Lysosomal Glycoproteins, specific glycosidases followed by a western blot analysis of a protein of interest provides a way to determine which types of glycans are present in the protein by comparing the gel mobility before and after treatment.

Derogate

会犯错误

Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells,c point, thereby the ratio can be used to determine the lysosomal pH. A standard curve is obtained by equilibrating intralysosomal pH with extracellular pH using the ionophore nigericin. The protocol also includes information regarding procedures to induce lysosomal alkalinization and lysosomal membrane permeabilization.

PALL

Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics,al lipidome by quantitatively detecting hundreds of lipid species of diverse physicochemical properties. We describe here a detailed protocol that couples isolation of superparamagnetic iron dextran-loaded lysosomes from cultured mammalian cell lines with quantitative mass spectrometry-based shotgun lipidomics.

大骂

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