Titlebook: Lymphoma; Tim Illidge,Peter W. M. Johnson Book 2005 Humana Press 2005 DNA.biopsy.chemistry.leukemia.lymphoma

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Purification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins, immunomagnetic method to isolate and purify malignant B-cells suitable for in vitro analyses from lymph node biopsies. Methods to analyze the expression of Bcl-2 family proteins by immunoblotting also are described.

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Identification of Lymphoma-Associated Antigens Using SEREX,equenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full.

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Idiotype Gene Rescue in Follicular Lymphoma,utation takes place. It has become evident that many of the common B-cell tumors arise at this site and are able to respond to stimuli, which govern normal B-cells. In this chapter, we focus on the analysis of V genes in follicular lymphomas based on the experience in our laboratory and provide a detailed guide for this analysis.

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1543-1894 treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity

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Tim Illidge,Peter W. M. JohnsonIncludes supplementary material:

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Methods in Molecular Medicinehttp://image.papertrans.cn/l/image/589224.jpg

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https://doi.org/10.1385/1592599362DNA; biopsy; chemistry; leukemia; lymphoma