Titlebook: Lymphangiogenesis; Methods and Protocol Guillermo Oliver,Mark L. Kahn Book 2018 Springer Science+Business Media, LLC, part of Springer Natu

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Guillermo Oliver,Mark L. KahnIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/589192.jpg

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Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function, immunofluorescent staining and visualization of valve organization, quantification of main morphological parameters such as valve size and leaflet length, and the quantitative assessment of functional properties of adult valves using back-leak and closure tests.

Affiliation

Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculatl cells and fibroblasts in their native matrix, without the use of synthetic scaffolds or exogenous factors. The tissue is stable over many weeks and accurately recapitulates features of human dermal lymphatic microvasculature.

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Visualization and Tools for Analysis of Zebrafish Lymphatic Development,gh resolution, staged imaging of lymphatic networks, how lymphangiogenesis can be reliably quantified and how immunofluorescence can reveal lymphatic signaling and differentiation. These methods offer researchers the opportunity to experimentally dissect developmental lymphangiogenesis with outstanding resolution.

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Imaging of Endothelial Cell Dynamic Behavior in Zebrafish,e most common imaging and quantifications challenges faced when acquiring and quantifying endothelial cell dynamic behavior in vivo..We intend this section to serve as an experience-based imaging primer for scientists interested in endothelial cell imaging in the zebrafish embryo.

Antecedent

Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis,alysis of morphology and structure, (3) lymphatic clearance assay for functional quantification, (4) whole-mount imaging with tissue clearing for confocal imaging, and (5) postmortem lymphangiography. These procedures allow for identification of morphological and functional abnormalities in both preexisting and newly formed lymphatic vessels.