Titlebook: Live Cell Imaging; Methods and Protocol Dmitri B. Papkovsky Book 2010 Humana Press 2010 Data analysis algorithms.Multi-mode microscopes.Mul

Resistance

Design of Fluorescent Fusion Protein Probeser becomes altered by conformational changes of a fused sensory protein in response to a cellular event. A structure-based approach can be taken to design probes better with improved dynamic ranges by computationally modeling conformational changes and predicting FRET efficiency changes of candidate

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Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cellsynitrite or hypochlorous acid is implicated in a broad array of human pathologies including vascular, immunological, and neurodegenerative diseases. However, unambiguous detection of these reactive oxygen species has been relatively difficult due to their short biological half-lives and multiple rea

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Veneer

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Imaging of Mitotic Cell Division and Apoptotic Intra-Nuclear Processes in Multicolor. Mitotic events from prophase to telophase are well defined by morphology or movement of chromatin, nuclear envelope, centrosomes, and/or spindles. To paint or simultaneously visualize these mitotic subcellular structures, we have been using ECFP-histone H3 for chromatin and chromosomes, EGFP-Auror

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lymphoma

Venules

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慢慢流出

Imaging and Analysis of Three-Dimensional Cell Culture Modelsectural microenvironment of natural tissue, compared to standard two-dimensional cultures. Microscopy techniques that function well for thin, optically transparent cultures, however, are poorly suited for imaging 3D cell cultures. Three-dimensional cultures may be thick and highly scattering, preven