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Titlebook: Live Cell Imaging; Methods and Protocol Dmitri B. Papkovsky Book 2010 Humana Press 2010 Data analysis algorithms.Multi-mode microscopes.Mul

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书目名称Live Cell Imaging
副标题Methods and Protocol
编辑Dmitri B. Papkovsky
视频video
概述Provides an easily accessible reference volume for live cell imaging written by leading researchers in the field.Details the analysis of a number of key biological parameters and processes on the scal
丛书名称Methods in Molecular Biology
图书封面Titlebook: Live Cell Imaging; Methods and Protocol Dmitri B. Papkovsky Book 2010 Humana Press 2010 Data analysis algorithms.Multi-mode microscopes.Mul
描述.Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In .Live Cell Imaging: Methods and Protocols., a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful .Methods in Molecular Biology.™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Live Cell Imaging: Met
出版日期Book 2010
关键词Data analysis algorithms; Multi-mode microscopes; Multi-photon excitation; Optoelectronics; Probes; Sampl
版次1
doihttps://doi.org/10.1007/978-1-60761-404-3
isbn_softcover978-1-4939-6140-5
isbn_ebook978-1-60761-404-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2010
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Design of Fluorescent Fusion Protein Probesy for real-time monitoring of cellular events within live cells by tracking changes in FRET efficiency. Stimulants can be used to trigger a range of cellular events including Ca. signaling, apoptosis, and subcellular translocations.
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Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cellseuronal cells under oxygen-glucose deprivation (OGD) conditions has been visualized for the first time by utilizing . probe, whilst the endogenous production of hypochlorous acid in macrophage cells upon stimulation with LPS, IFN-γ, and PMA has been imaged by utilizing . probe.
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Monitoring of Cellular Responses to Hypoxiabunits were labeled by fusing them to fluorescent proteins. Fluorescence resonance energy transfer (FRET) was used to determine the interaction of both subunits in living cells by confocal microscopy.
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Book 2010ology.™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Live Cell Imaging: Met
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Christian A. Wurm,Daniel Neumann,Roman Schmidt,Alexander Egner,Stefan Jakobs
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