Titlebook: Liposomes; Methods and Protocol Gerard G.M. D‘Souza,Hongwei Zhang Book 2023Latest edition The Editor(s) (if applicable) and The Author(s),

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Preparation of Doxorubicin Liposomes by Remote Loading Method,drug product in market. Since it was approved in 1995, DOXIL have been widely used in the treatment of various tumors. Several important technologies used in the development of doxorubicin liposomes, especially the remote loading technology, have an extremely important impact on the later liposome r

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Preparation and Physical Characterization of DNA Binding Cationic Liposomes, nucleic acids, peptides, and proteins. Cationic liposomes when complexed with DNA form a strong positively charged cationic liposome–DNA complex or lipoplex. The chapter discusses, primarily, the major preparation technique for cationic liposomes and its physical characterization, with a focus on S

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concentrate

Solid Lipid Nanoparticles for Drug Delivery, drug release or targeted delivery to the site of interest. Here we describe the preparation of solid lipid nanoparticles by forming a microemulsion at an elevated temperature which, upon cooling, yields a suspension of solid nanoparticles. This nanotemplate engineering method is inexpensive, reprod

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Stable Discoidal Bicelles: Formulation, Characterization, and Functions,sociated proteins. Recently, it has shown that bicelles can serve as nanocarriers to effectively deliver hydrophobic therapeutic molecules to cancer cells with a three- to ten-fold enhancement compared to that of liposomes of a chemically identical composition. In this chapter, detailed preparation

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Preparation and Characterization of Trastuzumab Fab-Conjugated Liposomes (Immunoliposomes),v), or new constructs such as nanobodies are commonly used instead of whole IgGs for reduced risk immunogenicity. Here we described the preparation and characterization of immunoliposome-containing trastuzumab Fabs on the surface. The targeting ligand Fab-PEG-DSPE was synthesized by site-specific co