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Titlebook: Lipoprotein Protocols; Jose M. Ordovas Book 1998 Humana Press 1998

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Assays of Lecithin Cholesterol Acyltransferase (LCAT) from the 2-. position of lecithin to the 3-hydroxy group of cholesterol. Ordinarily the reaction takes place mostly on the surface of high-density lipoproteins (HDL). In human plasma, this reaction is the major source of cholesteryl esters (CE) and has a crucial role in the remodeling of plasma lip
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Cellular Assays for Lipoprotein Receptorse receptor in culture. Receptor activity can also be evaluated indirectly by measuring the consequences of receptor-mediated cholesterol uptake on cellular cholesterol metabolism using enzyme assays such as those for acylCoA cholesterol acyltransferase (ACAT) and 3-hydroxy-3-methylglutaryl coenzyme
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Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins. With the arrival in the 1940s of the analytical ultracentrifuge, these particles could be separated from other plasma proteins as a discrete peak. During the following years, it was shown that this peak was made up of a wide spectrum of particle sizes and densities ranging from 0.92 to 1.21 g/mL (.).
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