Titlebook: Lipoprotein Protocols; Jose M. Ordovas Book 1998 Humana Press 1998

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Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresisor many different molecular biology procedures, and for the separation of large-size nucleic acid molecules (.); however, agarose gel electrophoresis is now becoming a tool of choice to separate small DNA-size fragments when the difference between the fragments of interest is greater than 1%, a task

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Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioartant as attempts are made to gain knowledge relating to how these altered conditions affect cells at the molecular level. Traditionally, an estimate of the copy number of a gene transcription product in a sample was made by employing traditional Northern blot, dot-blot hybridization, or solution hy

Seminar

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Preparation of Knockout Micehe other, it is produced in a large amount. Both conditions can be easily achieved using genetic engineering techniques. In the first case, the gene coding for the specific protein is inactivated (gene targeting) (.), whereas, in the second case, multiple copies of the gene are added (transgenesis)

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Separation of Apolipoproteins by Polyacrylamide Gel Electrophoresists involved in this technique can be modified, added, removed, or combined with the others to provide the optimal resolution for the specific interest of each researcher. Over the years, contmuous Improvements have been made to these techmques. More recently, the introduction of capillary electropho

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Isolation and Purification of Serum Lipoprotein (a) Using Lectin Affinityet al. discovered that Lp (a) correlated with coronary heart disease (CHD) in a case control study (.). Today, Lp (a) is considered by most as an independent risk factor in CHD. The mechanism by which it is atherogenic is not fully understood.

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Measurement of Lipoprotein Particleshat plasma lipoproteins could be viewed as a system of particles characterized by their apolipoprotein composition. This concept evolved in the proposal of using “apolipoprotem profiles” for typing dyslipoproteinemias. Along these lines, several recent reports have shown that certain apolipoprotein