Titlebook: Lipid-Protein Interactions; Methods and Protocol Jörg H. Kleinschmidt Book 2019Latest edition Springer Science+Business Media, LLC, part of

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Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Pres not have atomic-level resolution (unlike X-ray crystallography and NMR spectroscopy), but it has the great advantage of being applicable to both nonnative and native proteins in a wide range of solution conditions containing lipids and detergents. The development of synchrotron-radiation CD (SRCD

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Pedagogy

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,Förster Resonance Energy Transfer as a Tool for Quantification of Protein–Lipid Selectivity,id in its vicinity, from Förster resonance energy transfer methodologies. These allow a quantification of the effect, that is, the determination of the biasing in distribution of the lipid under study around the protein, as compared to its bulk membrane distribution, with advantages over established

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A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers, membranes. This chapter describes, in detail, the construction of a simple single molecule microscope, which includes: (1) a parts list, (2) temperature control, (3) an alignment procedure, (4) a calibration procedure, and (5) a procedure for measuring the mechanical stability of the instrument. It

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https://doi.org/10.1007/978-1-4939-9512-7lipidome scales; fluorescence microscopy; X-ray crystallography; Protein–Membrane Interactions; NMR Spec

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Jörg H. KleinschmidtIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts