Titlebook: Lipid Signaling Protocols; Mark G. Waugh Book 2016Latest edition Springer Science+Business Media New York 2016 cell function.ceramides.cho

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Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary lar membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.

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Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions,nt for vesicle trafficking and organelle function (Di Paolo and De Camilli, Nature 443(7112):651–657, 2006). The response triggered by these lipids is heavily dependent on the microenvironment in which they are found. HPLC analysis of labeled phosphoinositides allows quantification of the levels of

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Qualitative and Quantitative In Vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specifoduce the necessary yield and functionality in order to further characterize these enzymes. The outstanding versatility and sensitivity of this assay system are yet unmatched and are therefore currently considered the standard of the field.

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Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Micro-rich lipid raft structures. However, the intrinsically perturbing nature of fractionation methods makes the interpretation of such characterization subject to question, and indeed the existence and significance of lipid rafts remain controversial. Electron microscopic (EM) imaging of immunogold-lab

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Guidelines for the Use of Protein Domains in Acidic Phospholipid Imaging,ic lipid binding domains (LBDs) fused to fluorescent proteins. We discuss how to design such sensors, including the criteria for selecting the lipid binding domains of interest and to validate them. We also emphasize the care that must be taken during data analysis as well as the main limitations an

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