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Titlebook: Lipid Rafts; Thomas J. McIntosh Book 2007 Humana Press 2007 Lipid.X-ray.electron microscopy.fluorescence.magnetic resonance.microscopy.mol

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书目名称Lipid Rafts
编辑Thomas J. McIntosh
视频video
概述In-depth examination of sophisticated techniques used to study intact cell membranes.Proven, detailed biochemical, biophysical, and computational methods and protocols
丛书名称Methods in Molecular Biology
图书封面Titlebook: Lipid Rafts;  Thomas J. McIntosh Book 2007 Humana Press 2007 Lipid.X-ray.electron microscopy.fluorescence.magnetic resonance.microscopy.mol
描述In the past several years significant attention has been given to the analysis of the properties and functions of lateral microdomains (rafts) in biological membranes. As described in the overview chapter of this book, as well as in the introductions to many of the other chapters, there are many fundamental un- swered questions concerning the composition, structure, dynamics, and even the very existence of these membrane rafts. Therefore, a variety of sophisticated techniques have been used to study intact cell membranes, as well as model s- tems composed of specific lipids and proteins thought to be in rafts. The analyses of biological membranes have provided detailed data on intact microdomains, and the complementary studies of model systems have given critical insights on the roles of specific lipid-lipid and lipid-protein interactions in raft formation and function. The chapters in this book provide detailed information on modern methods that are currently being employed to study lipid rafts.
出版日期Book 2007
关键词Lipid; X-ray; electron microscopy; fluorescence; magnetic resonance; microscopy; molecular biology; protein
版次1
doihttps://doi.org/10.1007/978-1-59745-513-8
isbn_softcover978-1-61737-744-0
isbn_ebook978-1-59745-513-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2007
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Electro-Formation and Fluorescence Microscopy of Giant Vesicles With Coexisting Liquid Phases,to distinguish solid (gel) and liquid domains. Numerous experimental artifacts associated with GUV preparation and viewing are discussed, including the effects of nonideal growth conditions and perturbations of fluorescent probes and other impurities.
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Book 2007ogical membranes. As described in the overview chapter of this book, as well as in the introductions to many of the other chapters, there are many fundamental un- swered questions concerning the composition, structure, dynamics, and even the very existence of these membrane rafts. Therefore, a varie
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1064-3745 ional methods and protocolsIn the past several years significant attention has been given to the analysis of the properties and functions of lateral microdomains (rafts) in biological membranes. As described in the overview chapter of this book, as well as in the introductions to many of the other c
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Fluorescence Recovery After Photobleaching Studies of Lipid Rafts,beling cells with fluorescent lipid analogs or with the lipid-binding toxin cholera toxin B-subunit, (3) depleting and loading cholesterol into cell membranes using methyl-β-cyclodextrin, and (4) performing and analyzing confocal FRAP measurements.
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Overview of Membrane Rafts,g the composition, size, dynamics, and stability of membrane rafts. These questions are currently being addressed by a number of sophisticated biophysical, biochemical, and computational methodologies.
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