Titlebook: Lipase and Phospholipase Protocols; Mark Doolittle,Karen Reue Book 1999 Humana Press 1999

Impugn

Determination of Plasmalogen-Selective Phospholipase A2 Activity by Radiochemical and Fluorometric Aol backbone. Found in all mammalian cells, these phospholipids are especially abundant in brain and heart (.). However, while choline plasmalogen is enriched in heart tissue, brain tissue is rich in ethanolamine plasmalogen. Despite their ubiquitous distribution, little is known about their role in

critique

antedate

无辜

Bile Salt-Activated Lipasetic enzymes during intestinal dietary fat digestion processes (.). Because of its wide substrate reactivity, BAL is involved not only in the digestion of the major dietary fat, triacylglycerol, but also other important minor dietary lipids such as fat-soluble vitamin esters and cholesterol esters. B

fleeting

Fermentation

SMART

Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipaseiacylglycerol to diacylglycerol (for a recent review on HSL, ...). It also catalyzes the hydrolysis of diacylglycerols and monoacylglycerols. However, a second enzyme, monoglyceride lipase, is required to obtain a complete degradation of monoacylglycerols (.). HSL is most abundantly expressed in adi

世俗

Lecithin-Cholesterol AcyltransferaseAT has an important physiological role in the maturation of nascent, discoidal high density lipoproteins (HDL) to mature, spherical HDL, through the generation of a cholesteryl ester (CE)-enriched core (.,.). The enzyme has also been implicated in the reverse cholesterol transport pathway, which res

任命

Purification of Lipases and Phospholipases by Heparin-Sepharose Chromatographyand electrostatic interactions of heparin with enzymes, growth factors, receptors and blood coagulation factors (.–.). The general scheme of heparin-Sepharose chromatography involves the covalent attachment of heparin to Sepharose. A crude tissue preparation is then applied to the column; only those

用不完

Large-Scale Lipoprotein Lipase Purification from Adipose TissueDL and chylomicrons. Following hydrolysis of the triglyceride core of lipoproteins, some of the LPL molecules remain associated with chylomicron remnants and intermediate density lipoproteins (IDL) derived from VLDL. There is now accumulating evidence that these lipase molecules may play a role in t