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Titlebook: Light Microscopy; Methods and Protocol Hélio Chiarini-Garcia,Rossana C. N. Melo Book 2011 Springer Science + Business Media, LLC 2011 Brigh

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Hélio Chiarini-Garcia,Gleydes Gambogi Parreira,Fernanda R.C.L. Almeidaeory of direct methods, Patterson techniques, isomorphous replacement and anomalous scattering, and treatments of the role of electron microscopy and diffraction in crystal structure determination, including applications of direct methods to electron crystallography. Part 3 deals with applications o
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Histological Processing of Teeth and Periodontal Tissues for Light Microscopy Analysisthe most morphological and morphometric evaluations. Besides, this routine protocol allows the use of serial sectioning for more specific techniques such as histochemical and immunohistochemical analyses, which are suitable for cellular constituent and extracellular matrix evaluation of teeth and pe
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Large Plant Samples: How to Process for GMA Embedding?thod for processing large plant specimens that avoids these problems by: (1) slowing the polymerization process through cooling in order to permit the penetration of hardener into the sample core and (2) increasing the hardener:GMA ratio to aid polymerization of the sample core.
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Fluorescence Immunohistochemistry in Combination with Differential Interference Contrast Microscopy n the connective-tissue fibers between the lingual epithelium and the lingual muscle. The DIC images revealed the keratinization of the stratified squamous cells of the lingual epithelium and, also, myogenesis beneath the connective tissue. In addition, immunoreactivity specific for CIII was also re
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Image Cytometry: Nuclear and Chromosomal DNA Quantificationed in our laboratory are described. Protocols shown here for human DNA ploidy determination and quantification of nuclear and chromosomal DNA content in plants could be used as described, or adapted for other studies.
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Nestin-Driven Green Fluorescent Protein as an Imaging Marker for Nascent Blood Vessels in Mouse Moden stimulated or inhibited by specific compounds in both tumors and Gelfoam.. These fluorescent models can be used to study the early events of angiogenesis and to quantitatively determine efficacy of antiangiogenesis compounds.
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