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Titlebook: Light Microscopy; Methods and Protocol Hélio Chiarini-Garcia,Rossana C. N. Melo Book 2011 Springer Science + Business Media, LLC 2011 Brigh

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书目名称Light Microscopy
副标题Methods and Protocol
编辑Hélio Chiarini-Garcia,Rossana C. N. Melo
视频video
概述Readily reproducible protocols for optimal histological observations under bright-field microscopy.Provides full description of plastic resin embedding methods for plant and animal research.Presents a
丛书名称Methods in Molecular Biology
图书封面Titlebook: Light Microscopy; Methods and Protocol Hélio Chiarini-Garcia,Rossana C. N. Melo Book 2011 Springer Science + Business Media, LLC 2011 Brigh
描述Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques,all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring theintriguing microscopic world of the cell.
出版日期Book 2011
关键词Bright-field microscopy; Microscope instrumentation; Plastic resin embedding methods; Sample preparatio
版次1
doihttps://doi.org/10.1007/978-1-60761-950-5
isbn_softcover978-1-4939-5713-2
isbn_ebook978-1-60761-950-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science + Business Media, LLC 2011
The information of publication is updating

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Glycol Methacrylate Embedding for Improved Morphological, Morphometrical, and Immunohistochemical Invantages than paraffin embedding. The GMA improves the histological, morphometrical, and immunohistochemical evaluations, mainly due to the accurate assessment of cytological details. This chapter focuses on our experience in the GMA processing and describes in detail the fixation, embedding, and st
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Histological Approaches to Study Tissue Parasitism During the Experimental , Infection histological approaches for an optimal visualization and quantification of . nests in the heart, the main target organ of the parasite. These analyses are important to evaluate the course of the infection in different experimental models and also can be used to investigate parasite colonization and
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Intravital Microscopy to Study Leukocyte Recruitment In Vivo trafficking easier. Using this technique, we can directly visualize and measure each step of the leukocyte recruitment paradigm, including leukocyte rolling flux, rolling velocity, adhesion, and emigration. Thus, it is possible to understand the process involved in leukocyte homing as well as the c
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