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Titlebook: Laser Capture Microdissection; Methods and Protocol Graeme I. Murray Book 2018Latest edition Springer Science+Business Media, LLC 2018 Path

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书目名称Laser Capture Microdissection
副标题Methods and Protocol
编辑Graeme I. Murray
视频video
概述Includes cutting-edge methods and protocols involving laser capture microdissection.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from th
丛书名称Methods in Molecular Biology
图书封面Titlebook: Laser Capture Microdissection; Methods and Protocol Graeme I. Murray Book 2018Latest edition Springer Science+Business Media, LLC 2018 Path
描述This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and comprehensive, .Laser Capture Microdissection: Methods and Protocols, Third Edition. seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques..
出版日期Book 2018Latest edition
关键词Pathology; Tissue-based laser microdissection; Next generation sequencing; Gene expression analysis; Mic
版次3
doihttps://doi.org/10.1007/978-1-4939-7558-7
isbn_softcover978-1-4939-8520-3
isbn_ebook978-1-4939-7558-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2018
The information of publication is updating

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Next-Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (F prognosis, as well as the identification of therapeutically relevant genetic alterations. However, the vast majority of studies analyzing tissue samples use DNA extracted from bulk tissue or only manually microdissected specimens. Laser-assisted microdissection offers the possibility of isolating m
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Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontanetype. The primary tumors morphologically present both epithelial and spindle-like cells, but metastases in lung parenchyma display only adenocarcinoma properties. In the study of molecular signatures, laser capture microdissection (LCM) on frozen tissue sections was used to separate the following re
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Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease,form molecular analyses. Celiac disease is a genetic autoimmune disorder where the ingestion of gluten leads to damage in the small intestine. Increased intraepithelial lymphocytes and the presence of the lamina propria inflammatory infiltrate of the duodenal mucosa is a common part of the disease.
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Laser Capture Microdissection and Isolation of High-Quality RNA from Frozen Endometrial Tissue,om laser capture microdissected frozen tissue is beset by problems arising from intrinsic tissue RNase activity. Herein, we describe an optimized staining/LCM/RNA extraction protocol developed for the isolation of epithelial RNA from frozen tissue sections using human endometrial cancer as a model t
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Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Line to phenotypic changes or external stimuli. We describe a method that overcomes the technical challenges associated with the application of Laser Capture Microdissection to undecalcified bone enabling us to collect high-quality bone tissue with maintained cellular morphology that is suitable for cr
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