Titlebook: LC-MS/MS in Proteomics; Methods and Applicat Pedro R. Cutillas,John F. Timms Book 2010 Springer Science+Business Media, LLC 2010 Biological

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In-Depth Analysis of Protein Phosphorylation by Multidimensional Ion Exchange Chromatography and Maslex pathophysiological processes requires methods that can be used to profile and quantify protein phosphorylation as comprehensively as possible. Here we present a detailed protocol to enrich phosphopeptides from total cell lysates in a form amenable to downstream analysis by mass spectrometry. Usi

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Analysis of Carbohydrates on Proteins by Offline Normal-Phase Liquid Chromatography MALDI-TOF/TOF-MSuctural isomers) that vary in their degree of occupancy at given glycosylation sites. Hence, the detailed characterization of such glycans can be a very demanding task. Liquid chromatography in combination with mass spectrometric techniques can provide a fast and sensitive tool for the analysis of t

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