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Titlebook: LC-MS/MS in Proteomics; Methods and Applicat Pedro R. Cutillas,John F. Timms Book 2010 Springer Science+Business Media, LLC 2010 Biological

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书目名称LC-MS/MS in Proteomics
副标题Methods and Applicat
编辑Pedro R. Cutillas,John F. Timms
视频videohttp://file.papertrans.cn/581/580057/580057.mp4
概述Examines current LC-MS technologies, instrumentation, applications, and analysis solutions used in quantitative proteomics.Details protocols for isobaric tagging, in vivo stable isotopic labelling, la
丛书名称Methods in Molecular Biology
图书封面Titlebook: LC-MS/MS in Proteomics; Methods and Applicat Pedro R. Cutillas,John F. Timms Book 2010 Springer Science+Business Media, LLC 2010 Biological
描述With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes.In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications.Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS.Written in the highly successful Methods in Molecular Biology™ se
出版日期Book 2010
关键词Biological fluids and clinical samples; Chromatography; Liquid chromatography; Mass Spectrometry; Post-t
版次1
doihttps://doi.org/10.1007/978-1-60761-780-8
isbn_softcover978-1-4939-5744-6
isbn_ebook978-1-60761-780-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2010
The information of publication is updating

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978-1-4939-5744-6Springer Science+Business Media, LLC 2010
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LC-MS/MS in Proteomics978-1-60761-780-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Approaches and Applications of Quantitative LC-MS for Proteomics and Activitomics to quantify the activity of signalling and metabolic pathways in a multiplex and comprehensive manner, i.e. as an ‘activitomic’ tool. Taking cancer research as an illustrative example of application, this review discusses the concepts of biochemical pathway analysis using LC-MS-based proteomic and activitomic techniques.
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Overview of Quantitative LC-MS Techniques for Proteomics and Activitomicsed to quantify post-translational modifications and metabolites that reflect biochemical pathway activity. This review discusses the different analytical techniques that use LC-MS for the quantification of proteins, their modifications and activities in a multiplex manner.
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Quantification of Proteins by Label-Free LC-MS/MSantitative studies require high numbers of non-pooled samples for increased statistical power. We present a descriptive protocol for label-free quantitation of proteins by LC-MS/MS that enables to obtain both quantitative and qualitative information in one study without the need to pool samples or label them.
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s of drug tests, since outcome expectations and perceived self-efficacy influence people’s actions and behaviors. The drug-testing programs of the U.S. military services illustrate the dramatic effects that can be achieved by use of random drug testing based on standardized testing procedures and ba
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