Titlebook: Kidney Research; Experimental Protoco Tim D. Hewitson,Nigel D. Toussaint,Edward R. Smith Book 2023Latest edition The Editor(s) (if applicab

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A “Kidney-on-the-Chip” Model Composed of Primary Human Tubular, Endothelial, and White Blood Cells using primary human cells and the Mimetas scaffold that aims to increase pathophysiological significance of in vitro culture and simultaneously allows for relatively high-throughput and easy handling.

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Magnetic Resonance Imaging (MRI) Analysis of Tissue Sodium Concentration in Chronic Kidney Diseasee sodium is calibrated against known sodium chloride aqueous concentrations. This method may be useful for investigating in vivo (patho-)physiological conditions associated with tissue sodium deposition and metabolism (including in relation to water regulation) to enlighten our understanding of sodium physiology.

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Book 2023Latest editionals and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols... ..Authoritative and cutting-edge, .Kidney Research: Experimental Protocols, Third Edition .aims to be comprehensive guide for researchers in the field. .

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Generating Human Glomeruli from Pluripotent Stem Cells for Disease Modelling and Drug Screeningds in vitro. These 3D glomeruli retain appropriate transcriptional profiles and can be generated from any individual. As isolated glomeruli, they have applicability for disease modelling and drug discovery.

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Standardized Protocol for the Preparation of Precision-Cut Kidney Slices: A Translational Model of Rfeature of PCKS is that the slices contain all cell types and acellular components of the whole organ in the original configuration while preserving cell-cell and cell-matrix interactions. In this chapter, we describe how to prepare PCKS and how the model can be implemented in fibrosis research.

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Quantitative Imaging of Podocyte Foot Processes in the Kidney Using Confocal and STED Microscopytored using most standard procedures used today (i.e., PFA fixed, fresh frozen, formalin-fixed and paraffin-embedded (FFPE)). We additionally introduce tools for quantitative image analysis of foot process morphology and foot process effacement.

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Introduction

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In Vitro Models to Evaluate Molecular Permeability of the Kidney Filtration Barrierroperties during passive diffusion and under applied pressure. These systems can serve as a platform to evaluate the molecular permeability of basement membrane systems using conditions that simulate normal or pathophysiological conditions.