Titlebook: Kidney Organogenesis; Methods and Protocol Seppo Vainio Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 nephro

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Seppo VainioIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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nugatory

bronchiole

OASIS

Dissociation of Tissues for Single-Cell Analysisof research. Projects are underway to use these techniques to create an atlas of the expressed genes in all cell types of the human body. Here we describe cold-active protease methods for single-cell dissociation of organs and tissues that better preserve the in vivo gene expression patterns.

Nebulizer

Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Cultureuse of animals to model human renal physiology is limited, but human-derived renal organoids offer an alternative, relevant system in culture. Here, we explain how the activity of specific transport systems can be assessed in renal organoid and organ culture, using a system illustrated mainly for mouse but that can be extended to human organoids.

CROAK

Gene Editing in 3D Cultured Nephron Progenitor Cell Linesn vitro have enabled wide applications in both basic and translational research. Here we describe the methods to derive NPC lines from mouse embryonic kidneys in a 3D culture format as well as gene editing in the NPC lines.

Audiometry

LAIR