Titlebook: Kenzan Method for Scaffold-Free Biofabrication; Koichi Nakayama Book 2021 Springer Nature Switzerland AG 2021 3D cell printing.Biofabricat

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,Development of a Compact Bio 3D Printer, “S-PIKE®”,nd its potential contribution to research. This chapter introduces the concept of equipment for automatic manufacturing of cell products in regenerative medicine and the functions required for Bio 3D printing using the Kenzan method.

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Book 2021 multicellular spheroid positioning for building three dimensional construct only made from cells. The book explains the basic principles and concepts of the microneedle-based (“Kenzan”) method of building surgically-implantable tissue constructs using robotic cell spheroid-based three-dimensional b

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Scaffold-Free Biofabrication for Articular Cartilage (and Subchondral Bone),s deposited into osteochondral defects in minipigs. Next, a study concerning implantation of a “mold”-generated construct comprised of allogeneic AT-MSCs into a rabbit model is introduced. Furthermore, osteochondral regeneration following the autologous implantation of two swine AT-MSC tubular const

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Artificial Trachea: Past, Present, and Future,ediatric cases. Scaffold-free artificial trachea made using the patient’s own cells could resolve some of these issues. Artificial trachea created using the “Regenova” bio-3D printing system has been successfully transplanted into rats to replace native trachea, achieving survival for more than 1 ye

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Regeneration of the Diaphragm,owth. Histology revealed regeneration of the muscle structure, neovascularization, and neuronal networks within the reconstructed diaphragms. Our results show that our newly created cellular patches are an extremely safe and effective therapeutic strategy for repairing diaphragmatic defects.

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