Titlebook: Kala Azar in South Asia; Current Status and S E. Noiri,T.K. Jha Book 2016Latest edition Springer International Publishing 2016 Neglected Tr

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Challenges in the Diagnosis of Visceral Leishmaniasis on the Indian Subcontinents on the Indian subcontinent—a group that has limited access to proper health care. Untreated, kala-azar is almost always fatal, and the drugs currently in use are quite toxic. Confirmation of diagnosis before starting therapy is therefore crucial. Early diagnosis and treatment are a key strategy of

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CRAB

Antibody Capture Direct Agglutination Test (abcDAT) for Diagnosis of Visceral Leishmaniasis with Uri-human IgG. Urine samples are applied to the plate and then colored . promastigotes are added serving as direct agglutination test (DAT) antigens. If the urine contains IgG to ., the DAT antigen attaches to the plate; sediment will be observed if there is no specific IgG. Among 56 urine samples from

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Loop-Mediated Isothermal Amplification ( LAMP ): Molecular Diagnosis for the Field Survey of Viscerat features rapid reaction time, accuracy, cost-effect and results that can be determined with the naked eye. The most advantage of this method is that DNA amplification occurs at a constant temperature, usually between 60 and 65 °C, therefore sophisticated equipments are unnecessary. It can be easy

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Applicability of Multiplex Real-Time PCR to Visceral Leishmaniasisdered one of the most neglected tropical diseases in the Old World. Rapid and accurate diagnosis is needed to provide appropriate treatment to VL patients. Identification of the species that cause VL is useful to develop an epidemiology of leishmaniasis and reveal the biology of each species of .. H