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Titlebook: Kupffer Cells; Methods and Protocol Myriam Aouadi,Valerio Azzimato Book 2020 Springer Science+Business Media, LLC, part of Springer Nature

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发表于 2025-3-21 16:12:35 | 显示全部楼层 |阅读模式
书目名称Kupffer Cells
副标题Methods and Protocol
编辑Myriam Aouadi,Valerio Azzimato
视频videohttp://file.papertrans.cn/542/541270/541270.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Kupffer Cells; Methods and Protocol Myriam Aouadi,Valerio Azzimato Book 2020 Springer Science+Business Media, LLC, part of Springer Nature
描述.This volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ..  ..Authoritative and cutting-edge, .Kupffer Cells: Methods and Protocols  .aims to ensure successful investigation of this growing field. .
出版日期Book 2020
关键词steato-hepatitis; Mass Cytometry; electron microscopy; immunofluorescence; DNA sequencing
版次1
doihttps://doi.org/10.1007/978-1-0716-0704-6
isbn_softcover978-1-0716-0706-0
isbn_ebook978-1-0716-0704-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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发表于 2025-3-21 23:31:01 | 显示全部楼层
Kupffer Cell mRNA Sequencing,we describe a protocol to isolate and prepare cDNA libraries for mRNA sequencing of murine liver macrophages. Using mRNA sequencing to study the gene expression of macrophages in the liver provides a great tool to study the various functions of these cells in the regulation of homeostasis and immunity.
发表于 2025-3-22 04:08:46 | 显示全部楼层
Kupffer Cell Characterization by Mass Cytometry,etry offers a good compromise, allowing for the monitoring of a representative set of markers (up to 40) in a simple experiment. Herein, we describe a straightforward experimental and analysis workflow for Kupffer cell characterization by mass cytometry.
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发表于 2025-3-22 12:33:23 | 显示全部楼层
Kupffer Cell and Monocyte-Derived Macrophage Identification by Immunofluorescence on Formalin-Fixednction between Kupffer cells and monocyte-derived macrophages present on formalin-fixed, paraffin-embedded mouse liver samples. This protocol has been optimized for its reproducibility, reliability, and simplicity.
发表于 2025-3-22 14:32:02 | 显示全部楼层
Book 2020topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ..  ..Authoritative and cutting-edge, .Kupffer Cells: Methods and Protocols  .aims to ensure successful investigation of this growing field. .
发表于 2025-3-22 17:33:33 | 显示全部楼层
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发表于 2025-3-23 04:12:51 | 显示全部楼层
Liver Macrophage Isolation by Flow Cytometry Sorting,SH), and type 2 diabetes (T2D). Described herein is a protocol to isolate liver macrophages by FACS for downstream molecular analysis. Such studies of macrophages are important for elucidating their role in the pathogenesis of these metabolic diseases.
发表于 2025-3-23 08:22:58 | 显示全部楼层
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