Titlebook: Ion Channels; Methods and Protocol James D. Stockand,Mark S. Shapiro Book 2006 Humana Press 2006 Escherichia coli.Lipid.Vivo.molecular biol

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Book 2006eterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies.

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A Simple In Vivo Method for Assessing Changes of Membrane-Bound Ion Channel Density in , Oocytesent. This assay is linear over a wide range of channel expression levels and provides a simple cost-effective way of monitoring changes of membrane-bound channel density. Moreover, when the heterologous proteins poorly express at the plasma membrane, this method becomes advantageous to complex biochemical cell fractionation.

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KCNQ1 K+ Channel—Mediated Cardiac Channelopathiesng QT syndrome, characterized by prolonged QT interval, syncopes, and sudden death. Patch clamp and immunofluorescence techniques are instrumental for characterization of the molecular mechanisms responsible for the altered function of KCNQ1 and its partners.

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Tissue-Specific Transgenic and Knockout Miceng QT syndrome, characterized by prolonged QT interval, syncopes, and sudden death. Patch clamp and immunofluorescence techniques are instrumental for characterization of the molecular mechanisms responsible for the altered function of KCNQ1 and its partners.

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Probing the Effects of Phosphoinositides on Ion ChannelsK . channel, voltage-gated K . and Ca . channels, transient receptor potential channels, and intracellular inositol-1,4,5-trisphos-phate receptor ion channels. Discussed here are several methods for studying regulation of ion channels by phospholipids.

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Preparation of Cortical Brain Slices for Electrophysiological Recordingiderable functional differences in ion channel function within subregions of single cells. These findings have greatly increased understanding of neuronal computation. This chapter presents methods for obtaining high-quality brain slices developed to aid visualization of small neuronal structures using differential interference contrast microscopy

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Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.