Titlebook: Ion Channels; Methods and Protocol James D. Stockand,Mark S. Shapiro Book 2006 Humana Press 2006 Escherichia coli.Lipid.Vivo.molecular biol

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Tissue-Specific Transgenic and Knockout Miceciates with different β-subunits of the KCNE protein family. In the human heart, KCNQ1 associates with KCNE1 to generate the .. current characterized by its slow activation and deactivation kinetics. Mutations in either KCNQ1 or KCNE1 are responsible for at least four channelopathies that lead to ca

floodgate

白杨

大炮

Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression Systemtate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na. channel heterologously expressed in Chinese hamster ovary cells.

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James D. Stockand,Mark S. ShapiroIncludes supplementary material:

conjunctiva

hemoglobin

1064-3745 e their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy

赏钱

Overexpression of Proteins in Neurons Using Replication-Deficient Virusiological techniques to assess the effects of overexpressing wild-type or mutant proteins in neurons. Here, a general protocol for the production of replicationdeficient Semliki Forest virus constructs directing the overexpression of proteins of interest in cultured mammalian neurons is described.

Tracheotomy

Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using tensitive and blocker-insensitive subunits are coexpressed in a heterologous expression system. Details related to the expression of wild type and mutant ENaCs in . oocytes and the examination of blocker sensitivity by two-electrode voltage clamp, as well as analysis of data are provided.

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