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Titlebook: Intrinsically Disordered Proteins; Methods and Protocol Birthe B. Kragelund,Karen Skriver Book 2020 Springer Science+Business Media, LLC, p

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Determining , of IDPs from SAXS DataFranke et al., J Appl Crystallogr 50:1212–1225, 2017) has been used for the evaluation, and throughout the chapter, different examples will be given to illustrate the discussed phenomena, as well as the pros and cons of using the different approaches.
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https://doi.org/10.1007/978-1-0716-0524-0E; coli expression; post-translational modification; NMR; SAXS data; mass spectrometry
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Birthe B. Kragelund,Karen SkriverIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tract information. Here, we present a protocol to prepare monomeric samples of isotope-enriched short helical polyQ peptides based on the sequence of the androgen receptor (AR) suitable for NMR characterization and suggest different ways to adapt it for the production and monomerization of other relative
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