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Titlebook: Intrinsically Disordered Proteins; Methods and Protocol Birthe B. Kragelund,Karen Skriver Book 2020 Springer Science+Business Media, LLC, p

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发表于 2025-3-21 19:41:37 | 显示全部楼层 |阅读模式
书目名称Intrinsically Disordered Proteins
副标题Methods and Protocol
编辑Birthe B. Kragelund,Karen Skriver
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Intrinsically Disordered Proteins; Methods and Protocol Birthe B. Kragelund,Karen Skriver Book 2020 Springer Science+Business Media, LLC, p
描述The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Intrinsically Disordered Proteins: Methods and Protocols .aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules..Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com..Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..
出版日期Book 2020
关键词E; coli expression; post-translational modification; NMR; SAXS data; mass spectrometry
版次1
doihttps://doi.org/10.1007/978-1-0716-0524-0
isbn_softcover978-1-0716-0526-4
isbn_ebook978-1-0716-0524-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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发表于 2025-3-21 21:53:26 | 显示全部楼层
Exploring Protein Intrinsic Disorder with MobiDBrder predictions from protein sequences. It provides an easy-to-use web server to visualize and explore disorder information. This chapter describes the data available in MobiDB, emphasizing how to use and access the intrinsic disorder data. MobiDB is available at URL ..
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Computational Prediction of Disordered Protein Motifs Using SLiMSuite and online server examples are provided. Detailed notes provide additional advice on different applications of SLiMSuite, including batch running of multiple datasets and conservation masking using alignments of predicted orthologues.
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Expression and Purification of an Intrinsically Disordered Protein hexahistidine sequence (His-tag). This vector allows for the SUMO-AtLEA 3-2 fusion protein to be purified using Ni-affinity chromatography and, through the use of ubiquitin-like-specific protease 1 (Ulp1, a SUMO protease), results in an AtLEA 3-2 with a native N-terminus. We also describe the expression and purification of Ulp1 itself.
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Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopyabelling of the produced protein is defined by the chosen amino acids during reaction setup. Here we describe how to set up production and isotopic labelling of small intrinsically disordered proteins (IDPs) for NMR spectroscopy applications using an .-based CFPS system in batch mode.
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