Titlebook: Intrinsically Disordered Protein Analysis; Volume 2, Methods an Vladimir N. Uversky,A. Keith Dunker Book 2012 Springer Science+Business Med

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Dynamic and Static Light Scattering of Intrinsically Disordered Proteinsred proteins (IDPs), can be obtained from light scattering measurements. In this chapter, we discuss the physical basis of light scattering, experimental techniques, sample treatment, and data evaluation with special emphasis on studies on proteins. Static light scattering (SLS) is capable of measur

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Estimation of Intrinsically Disordered Protein Shape and Time-Averaged Apparent Hydration in Native scattering) is a useful methodology to characterize hydrodynamic properties of macromolecules, including intrinsically disordered proteins. The time-averaged apparent hydration and the shape factor of proteins can be estimated from the measured parameters (molecular mass, intrinsic viscosity, hydro

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Denaturant-Induced Conformational Transitions in Intrinsically Disordered Proteins also drive atypical responses of IDPs to changes in their environment. Among several specific features, the conformational behavior of IDPs is characterized by the low cooperativity (or the complete lack thereof) of the denaturant-induced unfolding. In fact, the denaturant-induced unfolding of nati

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Analyzing Temperature-Induced Transitions in Disordered Proteins by NMR Spectroscopy and Secondary Cn crucial for the understanding of the conformational properties of disordered proteins and is increasingly used to probe their conformational ensembles. Compared to folded proteins, disordered proteins are more malleable and more easily perturbed by environmental factors. Accordingly, the experimen

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Application of Confocal Single-Molecule FRET to Intrinsically Disordered Proteinslecules. A particularly versatile method is highly sensitive fluorescence detection. In combination with Förster resonance energy transfer (FRET), distances and conformational dynamics can be investigated in single molecules. This chapter introduces the practical aspects of applying confocal single-molecule FRET experiments to the study of IDPs.

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