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Titlebook: Intrinsically Disordered Protein Analysis; Volume 2, Methods an Vladimir N. Uversky,A. Keith Dunker Book 2012 Springer Science+Business Med

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书目名称Intrinsically Disordered Protein Analysis
副标题Volume 2, Methods an
编辑Vladimir N. Uversky,A. Keith Dunker
视频video
概述Details experimental methods used to identify IDP‘s and to analyze thier stucture.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
丛书名称Methods in Molecular Biology
图书封面Titlebook: Intrinsically Disordered Protein Analysis; Volume 2, Methods an Vladimir N. Uversky,A. Keith Dunker Book 2012 Springer Science+Business Med
描述.Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins. Structural biologists now recognize that the functional diversity provided by disordered regions complements the functional repertoire of ordered protein regions. In .Intrinsically Disordered Protein. Analysis: Methods and Experimental Tools., .expert researchers explore. .the high abundance of IDPs in various organisms, their unique structural features, numerous functions, and crucial associations with different diseases. .Volume 2 .includes sections on single molecule techniques, methods to assess protein size and shape, analyzing conformational behavior, mass-spectrometry, expression and purification of IDP’s. Written in the highly successful .Methods in Molecular Biology.™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory..Thorough and intuitive,. .Intrinsically Disordered Protein. Analysis: Methods and Experimental Tools. helps scientists further their investigations of these fascinating and dynamic molecules..
出版日期Book 2012
关键词functional analysis; globular proteins; intrinsically disordered proteins; physico-chemical; protein fun
版次1
doihttps://doi.org/10.1007/978-1-4614-3704-8
isbn_softcover978-1-4939-4484-2
isbn_ebook978-1-4614-3704-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2012
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Unequivocal Single-Molecule Force Spectroscopy of Intrinsically Disordered Proteinsal polymorphism of several IDPs. Here, we describe a simple protocol to perform the nanomechanical analysis of IDPs using this new strategy, a procedure that in principle can also be followed for the nanomechanical analysis of any protein.
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Dynamic and Static Light Scattering of Intrinsically Disordered Proteinsnsion or compaction of protein molecules. This is especially important for IDPs, which can be recognized and characterized by comparing the measured Stokes radii with those calculated for particular reference states, such as the compactly folded and the fully unfolded states. The combined applicatio
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