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Titlebook: Intrinsically Disordered Protein Analysis; Volume 1, Methods a Vladimir N. Uversky,A. Keith Dunker Book 2012 Springer Science+Business Med

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书目名称Intrinsically Disordered Protein Analysis
副标题Volume 1, Methods a
编辑Vladimir N. Uversky,A. Keith Dunker
视频video
概述Details experimental methods used to identify IDP‘s and to analyze their stucture.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
丛书名称Methods in Molecular Biology
图书封面Titlebook: Intrinsically Disordered Protein Analysis; Volume 1,  Methods a Vladimir N. Uversky,A. Keith Dunker Book 2012 Springer Science+Business Med
描述.Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins. Structural biologists now recognize that the functional diversity provided by disordered regions complements the functional repertoire of ordered protein regions.. .In .Intrinsically Disordered Protein. Analysis :Methods and Experimental Tools,. expert researchers explore. .the high abundance of IDPs in various organisms, their unique structural features, numerous functions, and crucial associations with different diseases. .Volume 1. includes sections on assessing IDPs in the living cell,NMR based techniques, vibrational spectroscopy, and other spectroscopic techniques. Written in the highly successful .Methods in Molecular Biology.™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.. .Thorough and intuitive,. .Intrinsically Disordered Protein .Analysis: Methods and Experimental Tools. helps scientists further their investigations of these fascinating and dynamic molecules. . .
出版日期Book 2012
关键词IDPs; disorder; functional properties; globular proteins; physico-chemical; structural properties; unfolde
版次1
doihttps://doi.org/10.1007/978-1-61779-927-3
isbn_softcover978-1-4939-6230-3
isbn_ebook978-1-61779-927-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2012
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In-Cell NMR in Mammalian Cells: Part 1, tools to characterize the structural and functional characteristics of IDPs inside mammalian cells are of particular interest. Moving from bacterial and amphibian in-cell NMR experiments to mammalian systems offers the unique opportunity to advance our knowledge about general IDP properties in nat
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In-Cell NMR in Mammalian Cells: Part 2pore-forming bacterial toxins. In the course of this procedure, mammalian cell membranes are permeated for short periods of time in order to enable the influx of exogenous proteins via a concentration gradient between the outside and the inside of the targeted “host” cells. In contrast to CPP-mediat
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Measurement and Analysis of NMR Residual Dipolar Couplings for the Study of Intrinsically Disordereds them to sample more efficiently their surroundings and thereby increase the probability of interaction with one or several different biological partners. Due to their high flexibility, IDPs cannot be represented by a single, three-dimensional structure; rather, an ensemble description can be invok
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