Titlebook: Innate Lymphoid Cells; Methods and Protocol Shoba Amarnath Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Dev

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Protocols for Studying Murine ILC Development,one marrow, their phenotype, and their potential to develop into mature ILC. These methods make up the foundation of in vivo investigations into ILC development, and we hope these thorough protocols and associated notes facilitate additional, high-quality inquiries into this fascinating field.

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Determining Coreceptor Expression and Function in Murine ILC2 Through Flow Cytometry Characterizati provide an experimental framework to study the function of coreceptors expressed on tissue ILC2. In brief, we describe flow cytometric analysis of the coreceptor of interest, the isolation and culture of mouse pulmonary ILC2 and splenic T cells, as well as approaches to manipulate their coculture. Finally, downstream readout options are outlined.

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Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells,oxycholesterol, which is mediated by cell surface-expressed GPR183. A similar strategy can be employed to test the role of other GPCRs in mediating the migration of ILCs toward other chemotactic ligands.

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Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease,on of human ILCs and intracellular cytokine expression using flow cytometry. We include protocols for isolating human peripheral blood and colonic lamina propria mononuclear cells essential for evaluating human IBD specimens.

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