Titlebook: Innate Lymphoid Cells; Methods and Protocol Shoba Amarnath Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Dev

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Determining Coreceptor Expression and Function in Murine ILC2 Through Flow Cytometry Characterizatiammation. Their low frequency in vivo during steady state condition may complicate research on the cells. During type 2 biased immune responses they are activated, increase in frequency and release cytokines as well as regulate T cell functions through direct interactions including MHC class II–T ce

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Protocol for Determining the Effect of Neuroendocrine Hormones on Murine ILC Function,mune response. It was demonstrated that endogenous glucocorticoids control the function of innate lymphoid cells (ILCs), and this regulatory mechanism is both cell type- and tissue-specific and is required for host protection during infections. We describe here how to analyze in vitro the effects of

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Isolation, Culture, and Induction of Plasticity in ILC2s,her immune cell populations and are primarily defined by lack of expression of markers associated with other immune cell lineages and are predominantly found in mucosal tissues like the gut, lung and skin. They are classified into distinct subsets, ILC1, ILC2, and ILC3, which mirror subsets of CD4.

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Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoave shown that specific ILC3 subsets protect against enteric bacterial pathogens. Here, we describe a mouse model of oral infection by . (.) and several different methodologies to assess the severity of the infection. We also detail how ILC3 subsets can be isolated from the mouse small intestine and

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Isolation, Characterization, and Culture of Intestinal Intraepithelial Lymphocytes,ll compartments in the body. IEL are located within the intestinal epithelium and are the first immune cells in the gut to interact with the food, microbiota, and pathogens that the gut is continually exposed to. IEL can respond rapidly to external insults to protect the small intestinal epithelium

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Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment,e NK cells, which are important in eliciting immunity against intracellular pathogens; group 2 ILCs protect against parasitic helminths; and group 3 ILCs protect against extracellular pathogens. The role of ILCs in cancer immunity remains unclear. In this chapter, we discuss methods for isolating an

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