Titlebook: Innate DNA and RNA Recognition; Methods and Protocol Hans-Joachim Anders,Adriana Migliorini Book 2014 Springer Science+Business Media New Y

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MicroRNA Methodology: Advances in miRNA Technologiesain microRNA specific methodologies and critical steps of microRNA expression profiling, target gene identification, and functional confirmation of microRNA effects up to in vivo application of antagomirs.

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Expression Profiling by Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) amount of amplified specific sequence can be quantified at each stage of the PCR cycle. If investigated sequence is present in large number of copies in particular sample, amplification product is detected already in earlier cycles; if the sequence is rare, amplification is observed in later cycles

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Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosussus (SLE). Endogenous RNA- and DNA-containing autoantigens released from dying cells can engage Toll-like receptors (TLR) 7/8 and TLR9, respectively in a number of immune cell types, thereby promoting innate and adaptive immune responses. Mouse models of lupus reliably phenocopy many of the characte

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Bifunctional siRNAs for Tumor Therapyolecules can be generated synthetically by chemical synthesis or by in vitro transcription (. Chapter 2, Hornung et al.). Similar to viruses, they initiate an antiviral immune response, e.g., by stimulation of the immune system. Short, double-stranded RNA in the cytosol can also trigger the RNA inte

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Fanny Matheis,Robert Besch Ph.D.s including finance, marketing, medicine, biology, engineering, telecommunications, software, and security..Data Mining and Knowledge Discovery Handbook, Second Edition. is designed for research scientists, lib978-0-387-09823-4

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ed spelling errors. When, subsequently, testing text is examined for context-based spelling errors, the logic formulas and a portion of the prior text are used to analyze the case at hand and to pinpoint likely errors. Tests conducted on different text samples indicate that the method is effective f