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Titlebook: Innate DNA and RNA Recognition; Methods and Protocol Hans-Joachim Anders,Adriana Migliorini Book 2014 Springer Science+Business Media New Y

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发表于 2025-3-21 17:03:10 | 显示全部楼层 |阅读模式
书目名称Innate DNA and RNA Recognition
副标题Methods and Protocol
编辑Hans-Joachim Anders,Adriana Migliorini
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Innate DNA and RNA Recognition; Methods and Protocol Hans-Joachim Anders,Adriana Migliorini Book 2014 Springer Science+Business Media New Y
描述.Innate DNA and RNA Recognition: Method and Protocols. presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical,. Innate DNA and RNA Recognition: Method and Protocols. provides a resource for immunologists, molecular biologists, virologists, microbiologists and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources..
出版日期Book 2014
关键词aberrant activation; epigenetic DNA and RNA modifications; extracellular nuclear particles; immune tole
版次1
doihttps://doi.org/10.1007/978-1-4939-0882-0
isbn_softcover978-1-4939-4583-2
isbn_ebook978-1-4939-0882-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
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发表于 2025-3-21 21:28:06 | 显示全部楼层
1064-3745 ation advice from the experts.Innate DNA and RNA Recognition: Method and Protocols. presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions t
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Detection of RNA Modifications by HPLC Analysis and Competitive ELISAge can lead to random modifications in rRNA and messenger RNA (mRNA) that strongly impair functionality. RNA modifications have also been implicated in avoiding self-RNA recognition by the immune system or immune evasion by pathogens. Here, we describe the detection of RNA modifications by HPLC analysis and competitive ELISA.
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Analysis of Nucleic Acid-Induced Nonimmune Cell Death In Vitroo cell death. Here, we describe reliable and simple methods to quantify cell death caused by nucleic acid recognition. Additionally, we report two different methods to discriminate between two cell death modalities: apoptosis and necrosis.
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Isolation of Intratumoral Leukocytes of TLR-Stimulated Tumor-Bearing Miceysis (e.g., by flow cytometry) or cultured for functional in vitro studies. Here, we describe the isolation of intratumoral leukocytes by density gradient centrifugation. This technique can be used to isolate leukocytes from freshly dissected murine tumors.
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Viral Nucleic Acid Recognition in Human Nonimmune Cells: In Vitro Systemsemplify two viral infection models using cultured nonimmune cells and hepatitis C virus (HCV) or polyomavirus BK (BKV). In addition, we report the importance of siRNA technique in gene regulation in order to identify specific markers involved in innate antiviral responses in these cells.
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