书目名称 | Innate Antiviral Immunity | 副标题 | Methods and Protocol | 编辑 | Karen Mossman | 视频video | http://file.papertrans.cn/467/466580/466580.mp4 | 概述 | Includes cutting-edge methods and protocols for the study of innate antiviral immunity.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and gr | 出版日期 | Book 2017 | 关键词 | Virus-host interactions; Model organisms; Infected cells; Novel pathways; CRISPR | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-7237-1 | isbn_softcover | 978-1-4939-8426-8 | isbn_ebook | 978-1-4939-7237-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media LLC 2017 |
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