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Titlebook: Innate Antiviral Immunity; Methods and Protocol Karen Mossman Book 2017 Springer Science+Business Media LLC 2017 Virus-host interactions.Mo

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发表于 2025-3-21 18:04:24 | 显示全部楼层 |阅读模式
书目名称Innate Antiviral Immunity
副标题Methods and Protocol
编辑Karen Mossman
视频videohttp://file.papertrans.cn/467/466580/466580.mp4
概述Includes cutting-edge methods and protocols for the study of innate antiviral immunity.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from
丛书名称Methods in Molecular Biology
图书封面Titlebook: Innate Antiviral Immunity; Methods and Protocol Karen Mossman Book 2017 Springer Science+Business Media LLC 2017 Virus-host interactions.Mo
描述This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and gr
出版日期Book 2017
关键词Virus-host interactions; Model organisms; Infected cells; Novel pathways; CRISPR
版次1
doihttps://doi.org/10.1007/978-1-4939-7237-1
isbn_softcover978-1-4939-8426-8
isbn_ebook978-1-4939-7237-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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