Titlebook: Inflammation; Methods and Protocol Björn E. Clausen,Jon D. Laman Book 2017 Springer Science+Business Media LLC 2017 human IMID.characteriza

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Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteinssue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. T

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Subcutaneous and Sublingual Immunotherapy in a Mouse Model of Allergic Asthmath an eosinophilic inflammation of the airways, as well as airway hyper responsiveness and remodeling. The inhaled allergens trigger a type-2 inflammatory response with involvement of innate lymphoid cells (ILC2) and Th2 cells, resulting in high production of immunoglobulin E (IgE) antibodies. Conse

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Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mousred group 2 innate lymphoid cells (ILC2). Due to a lack of unique markers, the accurate phenotypic characterization and quantification of ILC2 requires a comprehensive panel of fluorescently labeled antibodies. The markers that are currently used to characterize ILC2 have not been standardized and o

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Induction and Analysis of Bronchus-Associated Lymphoid Tissuephoid structure supporting the efficient priming of T cells in the lung. To explore the mechanisms and instructive signals controlling BALT neogenesis we used both, a single dose of vaccinia virus MVA and repeated inhalations of heat-inactivated . (.). Intranasal administration of both pathogens ind

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Messenger RNA Sequencing of Rare Cell Populations in the Lung and Lung-Draining Lymph Nodes knowledge about the large diversity and heterogeneity of cells that participate in immunological responses in a tissue- and micromilieu-specific manner calls for advanced isolation and sequencing methods for the accurate quantification of gene expression in small cell populations and even individua

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Monitoring and Modulation of Inducible Foxp3+ Regulatory T-Cell Differentiation in the Lymph Nodes Dic (Tg) T cells is a powerful tool to study antigen-specific regulatory T-cell differentiation in lymphoid tissues in vivo. The kinetics and nature of the T-cell response largely depend on the route of antigen administration and degree of clonal competition. Here, we describe that adoptive transfer

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