Titlebook: Induced Pluripotent Stem (iPS) Cells; Methods and Protocol Kursad Turksen,Andras Nagy Book 2016 Springer Science+Business Media New York 20

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GRAIN

Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors, the detailed protocol for the efficient generation of integration-free iPS cells from peripheral blood mononuclear cells. With this optimized protocol, one can readily generate hundreds of iPS cell colonies from 1 ml of peripheral blood.

Minikin

Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogrammingactor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous . expression during the reprogramming of mouse fibroblasts into iPS cells.

Immunization

Generation and Characterization of Rat iPSCs,l for establishing in vitro disease model, development of new protocols for treatment of different diseases, and creating transgenic rat models. Here, we describe a simple adopted protocol for establishing rat iPSCs from different types of somatic cells including rat primary ear fibroblast (PEF) and primary bone marrow cells (BMC).

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时间等

Book 2016protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Induced Pluripotent Stem (iPS) Cells: Methods and Protocols. provides protocols that can be used by both experts and novices interested in stem cells. . .

结果

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