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Titlebook: Induced Pluripotent Stem (iPS) Cells; Methods and Protocol Kursad Turksen,Andras Nagy Book 2016 Springer Science+Business Media New York 20

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发表于 2025-3-21 18:20:55 | 显示全部楼层 |阅读模式
书目名称Induced Pluripotent Stem (iPS) Cells
副标题Methods and Protocol
编辑Kursad Turksen,Andras Nagy
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Induced Pluripotent Stem (iPS) Cells; Methods and Protocol Kursad Turksen,Andras Nagy Book 2016 Springer Science+Business Media New York 20
描述.This volume aims to be a collection of essential protocols in iPS cell field. .Induced Pluripotent Stem (iPS) Cells: Methods and Protocols. .guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Induced Pluripotent Stem (iPS) Cells: Methods and Protocols. provides protocols that can be used by both experts and novices interested in stem cells. . .
出版日期Book 2016
关键词differentiation; four transcription factors; iPS cells; lineage commitment; somatic cells; stem cell biol
版次1
doihttps://doi.org/10.1007/978-1-4939-3055-5
isbn_softcover978-1-4939-7981-3
isbn_ebook978-1-4939-3055-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
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Induced Pluripotent Stem (iPS) Cells978-1-4939-3055-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/463853.jpg
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The , Transposon as a Platform Technology for Somatic Cell Reprogramming Studies in Mouse, reprogramming systems. These protocols are highlighted with examples from recent studies as to how . has been, and continues to be, conducive to the dissection of reprogramming processes at the cellular and molecular levels.
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Computational Biology Methods for Characterization of Pluripotent Cells,se and explain line by line a software protocol coded in R-Bioconductor for pluripotency assessment based on the comparison of transcriptomics data of pluripotent cells with an ESC of reference. I provide advice for experimental design, warning about possible pitfalls, and guides for results interpr
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cGMP-Compliant Expansion of Human iPSC Cultures as Adherent Monolayers, unpredictable and rely on operator experience and expertise. This is problematic for the cell manufacturing process where operator time and process predictability are often determining cost drivers. We have adopted a human iPSC system using defined media and a recombinant substrate that employs cel
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