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Titlebook: In Vitro Toxicity Testing Protocols; Sheila O’Hare,Chris K. Atterwill Book 1995 Springer Science+Business Media New York 1995

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书目名称In Vitro Toxicity Testing Protocols
编辑Sheila O’Hare,Chris K. Atterwill
视频video
丛书名称Methods in Molecular Biology
图书封面Titlebook: In Vitro Toxicity Testing Protocols;  Sheila O’Hare,Chris K. Atterwill Book 1995 Springer Science+Business Media New York 1995
描述In vitro toxicology is one of the most rapidly expanding areas of biological research today. It is generally conceded that this is a result of pressure from the public for safer products and environmental conditions and, in these recessionary times, pressure from company accountants, who often perceive in vitro experimentation as a cheaper option than lawsuits, fines, and expensive remediation. Toxicologists themselves form one group whose vigor and influence on the evolution of in vitro toxicology is often underestimated. Many toxicologists have for some time been unhappy with the quantity (often a result of unnecessary duplication), the reliability, and the ethical circumstances of much animal experimentation. It is hoped that the present book, In Vitro Toxicity Testing Protocols, will play some part in enabling and encouraging the more widespread use of in vitro techniques. Since in vitro toxicity testing methodology is evolving at such a rapid rate, this book can of necessity provide only a "snapshot" of the major techniques in use at the present time. As with the other publications in Humana Press‘ Methods in Molecular Biology series, the aim has been to supply information on
出版日期Book 1995
版次1
doihttps://doi.org/10.1385/0896032825
isbn_softcover978-1-4899-4082-7
isbn_ebook978-1-59259-530-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 1995
The information of publication is updating

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Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicitydures allow relatively rapid assessment of different chemicals or their metabolites over a range of concentrations, using cells derived from a restricted source. The use of multiwell plates for the cultural astrocytes means that multiple samples can be analyzed with a high degree of statistical accu
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Cytotoxicity, DNA Fragmentation, and DNA Repair Synthesis in Primary Human Hepatocytesy in the extrapolation to humans of the results of toxicity studies carried out in laboratory animals or in in vitro systems employing as targets prokaryotes, lower eukaryotes, or mammalian cells. Growing evidence indicates that the most important cause of these species-related differences is the di
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The FRAME Cytotoxicity Test (Kenacid Blue)-L1 cells (an established cell-line, ATCC CCL92.1), when maintained in culture continuously divide and multiply over time. The basis of this test is that a cytotoxic chemical (regardless of site or mechanism of action) will interfere with this process and, thus, result in a reduction of the growth r
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Measurement of Cell Membrane Toxicity by Means of 2-Deoxy-D-Glucosed was first advanced to explain the toxic effects of heavy metals. The vital regulatory mechanisms inherent in the plasma membrane and its chemical composition make it susceptible to many toxic compounds (.). A toxic substance may cause structural alterations in the membrane by binding to or crossli
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SIRC Cytotoxicity Testll viability. A decrease in cell number, as measured by uptake of the dye Neutral Red, serves as an indicator of potential cytotoxicity. This test has been proposed as a potential replacement alternative for the Draize Eye Irritation test (.).
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