Titlebook: In Vitro Mutagenesis; Methods and Protocol Andrew Reeves Book 2017 Springer Science+Business Media New York 2017 gene and genome editing.fa

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书目名称In Vitro Mutagenesis影响因子(影响力)




书目名称In Vitro Mutagenesis影响因子(影响力)学科排名




书目名称In Vitro Mutagenesis网络公开度




书目名称In Vitro Mutagenesis网络公开度学科排名




书目名称In Vitro Mutagenesis被引频次




书目名称In Vitro Mutagenesis被引频次学科排名




书目名称In Vitro Mutagenesis年度引用




书目名称In Vitro Mutagenesis年度引用学科排名




书目名称In Vitro Mutagenesis读者反馈




书目名称In Vitro Mutagenesis读者反馈学科排名

阴谋

In Vitro Mutagenesis978-1-4939-6472-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

NAG

ACME

Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/463027.jpg

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exigent

All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells transient puromycin selection, to validate the mutation efficiency by Surveyor nuclease assay, and to perform off-target analyses. We show that our protocol enables highly efficient multiplex genome engineering even in hard-to-transfect HepG2 cells.

Eructation

lavish

In Silico Prediction of Deleteriousness for Nonsynonymous and Splice-Altering Single Nucleotide Varig predictions from multiple databases can facilitate the process. In this chapter, we describe the general steps of obtaining comprehensive predictions and annotations for large numbers of variants from dbNSFP, the first and probably the most widely used database of its kind.

Decibel

In Silico Methods for Analyzing Mutagenesis Targetsamics are then demonstrated to evaluate the energy landscape, or potential of mean force, along the chosen reaction coordinate. The potential of mean force identifies variations of the predominant structures among mutants that may affect function.

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