Titlebook: In Situ Hybridization Protocols; Ian A. Darby,Tim D. Hewitson Book 2006Latest edition Humana Press 2006 DNA.Microarray.Termination.electro

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Identification of Transplanted Human Cells in Animal Tissues,rious types, especially embryonic stem cells, reinforced this interest. Systematic studies are now being launched to define the biology of various stem cells, including after transplantation of cells in immunodeficient animals. This requires robust and unequivocal means to identify transplanted cell

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Histochemical Localization of Cell Proliferation Using , Hybridization for Histone mRNA,guish cells in the synthetic or S phase have tended to rely on the in vivo incorporation of tritiated thymidine or thymidine analogs such as bromodeoxyuridine. The necessity to pulse with these labels before retrieving tissue means that they cannot be used in humans and are not available retrospecti

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The Use of Combined Immunohistochemical Labeling and , Hybridization to Colocalize mRNA and Proteinuse in formalin- fixed, paraffin-embedded, or frozen tissue sections. This technique allows the combination of detecting mRNA by . hybridization with immunohistochemical detection of a protein product or a cell surface marker without using any radioactive procedures. This technique is ideal for use

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Semiquantitative , Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Poolymerase chain reaction is the ability to localize specific species of mRNA to a particular cell population in a heterocellular system. This consideration is important when studying gene expression, especially in areas of high cellular heterogeneity, such as the central nervous system, where the di

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