| 书目名称 | In Situ Hybridization Protocols |
| 编辑 | Ian A. Darby,Tim D. Hewitson |
| 视频video | |
| 概述 | Includes supplementary material: |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biol |
| 出版日期 | Book 2006Latest edition |
| 关键词 | DNA; Microarray; Termination; electron microscopy; gene expression; genes; hybridization; molecular biology |
| 版次 | 3 |
| doi | https://doi.org/10.1385/1597450073 |
| isbn_softcover | 978-1-61737-544-6 |
| isbn_ebook | 978-1-59745-007-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Humana Press 2006 |
发表于 2025-3-21 17:21:19
