Titlebook: In Situ Hybridization Protocols; K. H. Andy Choo Book Oct 19941st edition Humana Press 1995

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Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase basic unit is a monomer repeat of approx 170 basepair (bp) that contains both sequences conserved among the different chromosomes and variable regions. Blocks of consecutive monomers comprise a chromosome-specific higher-order repeat of up to several kilobases (kb) in size. For most human chromosom

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Primed In Situ (PRINS) Labeling of DNAdetection was possible, which to some extent made these techniques unsuited for routine purposes. Within the last decade, a number of nonradioactive detection methods have become available, resulting in a much more widespread use of . hybridization. These methods have become of great value, e.g., as

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Free Chromatin Mapping by FISHctrophoresis and blot-hybridization analysis, DNA sequencing, construction of somatic cell hybrid lines, . hybridization, as well as genetic (linkage) analysis. Because of their wide application, numerous improvements have been made in each of these areas in recent years, for example, the use of pul

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In Situ Hybridization of Meiotic Prophase Chromosomesis in part, a result of traditional fixation procedures that use alcohol, acetic acid, and/or relatively strong ionic conditions, all of which can cause chromosome condensation. The limit of resolution may be improved by using free chromatin or DNA-halo preparations as described in . and . of this v

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Strand-Specific Fluorescence In Situ Hybridization for Determining Orientation and Direction of DNA tation and strand direction of DNA sequences (.). Standard fluorescent . hybridization methods rely on nucleotide base pairing between a labeled probe and the complementary chromosomal target sequence. Both probe and target DNAs must be functionally single-stranded at the start of the procedure. Sin

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Gene Mapping Using 3H-Labeled Heterologous Probese cloned probe, labeled by a radioactive or fluorescent tag, hybridizes specifically to the single-stranded sequence(s) in the genome, and the location of the gene or sequence of interest on the chromosome is visualized by autoradiography or fluorography. Here we will discuss only radioactive . hybr